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Enzymatic and acid hydrolysis of Tetraselmis suecica for polysaccharide characterization

Paul Anastas, Julie Zimmerman and 2 other contributors

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    Publication Date
    DEC
    Journal
    BIORESOURCE TECHNOLOGY
    Location
    https://doi.org/10.1016/j.biortech.2014.09.048
    Citation
    Kermanshahi-Pour, A. ., Sommer, T. ., Anastas, P. ., & Zimmerman, J. . (2014). Enzymatic and acid hydrolysis of Tetraselmis suecica for polysaccharide characterization. BIORESOURCE TECHNOLOGY, 173, 415-421. https://doi.org/10.1016/j.biortech.2014.09.048 (Original work published 2024)

    Related YSE Profiles

    Faculty
    Paul Anastas

    Paul Anastas Teresa and H. John Heinz III Professor in the Practice of Chemistry for the Environment

    Faculty
    Julie Zimmerman

    Julie Zimmerman Vice Provost for Planetary Solutions, Professor of Green Engineering, Deputy Director for Research at Center for Green Chemistry and Green Engineering

    Abstract

    Carbohydrate composition of the marine microalgae, Tetraselmis suecica was characterized following acidic and enzymatic hydrolysis. Monitoring intracellular starch as a function of cultivation time at varying nitrate concentrations showed a maximum cellular starch content of 45% of dry biomass when grown under nitrate depleted conditions. Characterization of the cell wall methanolysates using GC/MS showed that the monosaccharide composition did not change in response to the nitrate concentration and consisted of 54% 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), 17% 3-deoxy-lyxo-2-heptulosaric acid (Dha), 21% galacturonic acid and 6% galactose. Presence of up to 5% Kdo in the dry weight of T. suecica established in this study demonstrates the potential of the cell wall of this species as a feedstock for Kdo, a sugar that is difficult to obtain by chemical synthesis and that has applications in medicinal chemistry. (c) 2014 Elsevier Ltd. All rights reserved.